![]() ![]() The genetic marker is a known DNA sequence or gene located on the chromosome which can be applied in the identification of individual species or organisms or we can use it in the identification of other genes or DNA sequences.īefore that let me give you a brief idea about how different genetic markers are developed. “The genetic markers are often known as DNA marker is a known DNA sequence located in the genome, used in the molecular genetics for identification of alteration or DNA sequence from the unknown DNA sample.” SCAR: Sequence characterized amplified region.RAPD: Random amplification of polymorphic DNA.AFLP: Amplified fragment length polymorphism.RFLP: Restriction fragment length polymorphism.SCAR (sequence characterized amplified region).AFLP (Amplified fragment length polymorphism).RFLP(Restriction fragment length polymorphism).In the present article, we will understand some common markers used in the molecular genetic technique along with their applications. For studying any kind of alterations (polymorphism). ![]() Thus from the definition, we can say that broadly, the Genetic markers are used, An alternative name for the technique is Cleaved Amplified Polymorphic Sequence (CAPS) assay.“Genetic markers are known DNA sequences which can be used for studying any kind of polymorphism”.Ī genetic marker is a known location on a chromosome used for the identification of individuals among and between species. Therefore, more samples can be analyzed in a shorter time. However, PCR can be used to amplify very small amounts of DNA, usually in 2-3 hours, to the levels required for RFLP analysis. Isolation of sufficient DNA for RFLP analysis is time consuming and labor intensive. Typically, in species with moderate to high polymorphism rates, two to four restriction endonucleases are used such as EcoRI
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